MxA transcripts with distinct first exons and modulation of gene expression levels by single-nucleotide polymorphisms in human bronchial epithelial cells
Identifieur interne : 001E58 ( Main/Exploration ); précédent : 001E57; suivant : 001E59MxA transcripts with distinct first exons and modulation of gene expression levels by single-nucleotide polymorphisms in human bronchial epithelial cells
Auteurs : Satoshi Noguchi [Japon] ; Minako Hijikata [Japon] ; Emi Hamano [Japon] ; Ikumi Matsushita [Japon] ; Hideyuki Ito [Japon] ; Jun Ohashi [Japon] ; Takahide Nagase [Japon] ; Naoto Keicho [Japon]Source :
- Immunogenetics [ 0093-7711 ] ; 2013-02-01.
English descriptors
- KwdEn :
Abstract
Abstract: Myxovirus resistance A (MxA) is a major interferon (IFN)-inducible antiviral protein. Promoter single-nucleotide polymorphisms (SNPs) of MxA near the IFN-stimulated response element (ISRE) have been frequently associated with various viral diseases, including emerging respiratory infections. We investigated the expression profile of MxA transcripts with distinct first exons in human bronchial epithelial cells. For primary culture, the bronchial epithelium was isolated from lung tissues with different genotypes, and total RNA was subjected to real-time reverse transcription polymerase chain reaction. The previously reported MxA transcript (T1) and a recently registered transcript with a distinct 5′ first exon (T0) were identified. IFN-β and polyinosinic–polycytidylic acid induced approximately 100-fold higher expression of the T1 transcript than that of the T0 transcript, which also had a potential ISRE motif near its transcription start site. Even without inducers, the T1 transcript accounted for approximately two thirds of the total expression of MxA, levels of which were significantly associated with its promoter and exon 1 SNPs (rs17000900, rs2071430, and rs464138). Our results suggest that MxA observed in respiratory viral infections is possibly dominated by the T1 transcript and partly influenced by relevant 5′ SNPs.
Url:
DOI: 10.1007/s00251-012-0663-8
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000831
- to stream Istex, to step Curation: 000831
- to stream Istex, to step Checkpoint: 000568
- to stream Main, to step Merge: 001E82
- to stream Main, to step Curation: 001E58
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">MxA transcripts with distinct first exons and modulation of gene expression levels by single-nucleotide polymorphisms in human bronchial epithelial cells</title><author><name sortKey="Noguchi, Satoshi" sort="Noguchi, Satoshi" uniqKey="Noguchi S" first="Satoshi" last="Noguchi">Satoshi Noguchi</name></author><author><name sortKey="Hijikata, Minako" sort="Hijikata, Minako" uniqKey="Hijikata M" first="Minako" last="Hijikata">Minako Hijikata</name></author><author><name sortKey="Hamano, Emi" sort="Hamano, Emi" uniqKey="Hamano E" first="Emi" last="Hamano">Emi Hamano</name></author><author><name sortKey="Matsushita, Ikumi" sort="Matsushita, Ikumi" uniqKey="Matsushita I" first="Ikumi" last="Matsushita">Ikumi Matsushita</name></author><author><name sortKey="Ito, Hideyuki" sort="Ito, Hideyuki" uniqKey="Ito H" first="Hideyuki" last="Ito">Hideyuki Ito</name></author><author><name sortKey="Ohashi, Jun" sort="Ohashi, Jun" uniqKey="Ohashi J" first="Jun" last="Ohashi">Jun Ohashi</name></author><author><name sortKey="Nagase, Takahide" sort="Nagase, Takahide" uniqKey="Nagase T" first="Takahide" last="Nagase">Takahide Nagase</name></author><author><name sortKey="Keicho, Naoto" sort="Keicho, Naoto" uniqKey="Keicho N" first="Naoto" last="Keicho">Naoto Keicho</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:A2260D448B85CDF8AE6C28A233C151B3928DC6B7</idno><date when="2012" year="2012">2012</date><idno type="doi">10.1007/s00251-012-0663-8</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-FF4M1K5R-F/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000831</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000831</idno><idno type="wicri:Area/Istex/Curation">000831</idno><idno type="wicri:Area/Istex/Checkpoint">000568</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000568</idno><idno type="wicri:doubleKey">0093-7711:2012:Noguchi S:mxa:transcripts:with</idno><idno type="wicri:Area/Main/Merge">001E82</idno><idno type="wicri:Area/Main/Curation">001E58</idno><idno type="wicri:Area/Main/Exploration">001E58</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">MxA transcripts with distinct first exons and modulation of gene expression levels by single-nucleotide polymorphisms in human bronchial epithelial cells</title><author><name sortKey="Noguchi, Satoshi" sort="Noguchi, Satoshi" uniqKey="Noguchi S" first="Satoshi" last="Noguchi">Satoshi Noguchi</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Respiratory Diseases, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku-ku, 162-8655, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Respiratory Medicine, University of Tokyo Hospital, 113-0033, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Hijikata, Minako" sort="Hijikata, Minako" uniqKey="Hijikata M" first="Minako" last="Hijikata">Minako Hijikata</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Respiratory Diseases, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku-ku, 162-8655, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Hamano, Emi" sort="Hamano, Emi" uniqKey="Hamano E" first="Emi" last="Hamano">Emi Hamano</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Respiratory Diseases, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku-ku, 162-8655, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Respiratory Medicine, University of Tokyo Hospital, 113-0033, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Matsushita, Ikumi" sort="Matsushita, Ikumi" uniqKey="Matsushita I" first="Ikumi" last="Matsushita">Ikumi Matsushita</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Respiratory Diseases, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku-ku, 162-8655, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Ito, Hideyuki" sort="Ito, Hideyuki" uniqKey="Ito H" first="Hideyuki" last="Ito">Hideyuki Ito</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Thoracic Surgery, National Center for Global Health and Medicine, 162-8655, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Ohashi, Jun" sort="Ohashi, Jun" uniqKey="Ohashi J" first="Jun" last="Ohashi">Jun Ohashi</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Molecular and Genetic Epidemiology, Faculty of Medicine, University of Tsukuba, 305-8575, Ibaraki</wicri:regionArea><wicri:noRegion>Ibaraki</wicri:noRegion></affiliation></author><author><name sortKey="Nagase, Takahide" sort="Nagase, Takahide" uniqKey="Nagase T" first="Takahide" last="Nagase">Takahide Nagase</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Respiratory Medicine, University of Tokyo Hospital, 113-0033, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Keicho, Naoto" sort="Keicho, Naoto" uniqKey="Keicho N" first="Naoto" last="Keicho">Naoto Keicho</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Respiratory Diseases, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku-ku, 162-8655, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Japon</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Immunogenetics</title><title level="j" type="abbrev">Immunogenetics</title><idno type="ISSN">0093-7711</idno><idno type="eISSN">1432-1211</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="2013-02-01">2013-02-01</date><biblScope unit="volume">65</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="107">107</biblScope><biblScope unit="page" to="114">114</biblScope></imprint><idno type="ISSN">0093-7711</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0093-7711</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Human bronchial epithelial cells</term><term>Myxovirus resistance A</term><term>Single-nucleotide polymorphism</term><term>Transcript variants</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Myxovirus resistance A (MxA) is a major interferon (IFN)-inducible antiviral protein. Promoter single-nucleotide polymorphisms (SNPs) of MxA near the IFN-stimulated response element (ISRE) have been frequently associated with various viral diseases, including emerging respiratory infections. We investigated the expression profile of MxA transcripts with distinct first exons in human bronchial epithelial cells. For primary culture, the bronchial epithelium was isolated from lung tissues with different genotypes, and total RNA was subjected to real-time reverse transcription polymerase chain reaction. The previously reported MxA transcript (T1) and a recently registered transcript with a distinct 5′ first exon (T0) were identified. IFN-β and polyinosinic–polycytidylic acid induced approximately 100-fold higher expression of the T1 transcript than that of the T0 transcript, which also had a potential ISRE motif near its transcription start site. Even without inducers, the T1 transcript accounted for approximately two thirds of the total expression of MxA, levels of which were significantly associated with its promoter and exon 1 SNPs (rs17000900, rs2071430, and rs464138). Our results suggest that MxA observed in respiratory viral infections is possibly dominated by the T1 transcript and partly influenced by relevant 5′ SNPs.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Noguchi, Satoshi" sort="Noguchi, Satoshi" uniqKey="Noguchi S" first="Satoshi" last="Noguchi">Satoshi Noguchi</name></region><name sortKey="Hamano, Emi" sort="Hamano, Emi" uniqKey="Hamano E" first="Emi" last="Hamano">Emi Hamano</name><name sortKey="Hamano, Emi" sort="Hamano, Emi" uniqKey="Hamano E" first="Emi" last="Hamano">Emi Hamano</name><name sortKey="Hijikata, Minako" sort="Hijikata, Minako" uniqKey="Hijikata M" first="Minako" last="Hijikata">Minako Hijikata</name><name sortKey="Ito, Hideyuki" sort="Ito, Hideyuki" uniqKey="Ito H" first="Hideyuki" last="Ito">Hideyuki Ito</name><name sortKey="Keicho, Naoto" sort="Keicho, Naoto" uniqKey="Keicho N" first="Naoto" last="Keicho">Naoto Keicho</name><name sortKey="Keicho, Naoto" sort="Keicho, Naoto" uniqKey="Keicho N" first="Naoto" last="Keicho">Naoto Keicho</name><name sortKey="Matsushita, Ikumi" sort="Matsushita, Ikumi" uniqKey="Matsushita I" first="Ikumi" last="Matsushita">Ikumi Matsushita</name><name sortKey="Nagase, Takahide" sort="Nagase, Takahide" uniqKey="Nagase T" first="Takahide" last="Nagase">Takahide Nagase</name><name sortKey="Noguchi, Satoshi" sort="Noguchi, Satoshi" uniqKey="Noguchi S" first="Satoshi" last="Noguchi">Satoshi Noguchi</name><name sortKey="Ohashi, Jun" sort="Ohashi, Jun" uniqKey="Ohashi J" first="Jun" last="Ohashi">Jun Ohashi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001E58 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001E58 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:A2260D448B85CDF8AE6C28A233C151B3928DC6B7
|texte= MxA transcripts with distinct first exons and modulation of gene expression levels by single-nucleotide polymorphisms in human bronchial epithelial cells
}}
| This area was generated with Dilib version V0.6.33. |  |